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KMID : 1024420120160030214
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Food Engineering Progress
2012 Volume.16 No. 3 p.214 ~ p.218
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Optimization of Lateral Flow Assay for the Dual-detection of Mycotoxins
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Moon Ji-Hye
Kim Gi-Young
Lee Sang-Dae
Park Saet-Byul
Kim Hyeok-Ju
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Abstract
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This study was performed to develop and optimize a rapid strip kit for the dual-detection of Aflatoxin B1 (AF) and Ochratoxin A (OTA). The strip kit is composed of sample pad, conjugation pad, nitrocellulose membrane, and absorbent pad. Manually spotted mycotoxin-BSA conjugates and anti-mouse IgG on nitrocellulose membrane were used as the test line and the control line, respectively. Conditions for rapid and easy detection of mycotoxins, conjugation between toxin-MAbs and nano Au particles, pretreatment method of pads, and composition of standards solutions were optimized. Feasibility of the strip kit to detect AF and OTA were evaluated with standard samples. Test results were acquired in 10 min and the detection limits were 100 ppm for AF and OTA, respectively.
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KEYWORD
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aflatoxin B1, ochratoxin A, lateral flow assay, mycotoxins, biosensor
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